Rebirth of the God-level Xueba

Yang Rui took out a Montblanc from the drawer, patted his head before writing, and said, "Go and make a copy first, lest I mess it up."

"It's okay, you can paddle as you like." Gu Qiang's attitude was extremely cute.

"It's better to make a copy, keep a record, and compare it later." Yang Rui insisted.

Gu Qiang had no choice but to copy it as he said, looking at the hundreds of pages of experimental design, feeling a little bit reluctant. It's not that he was reluctant to part with the experimental design he wrote, but that he was reluctant to part with copy paper and ink.

Today's copiers are all Japanese products, not to mention the expensive machines, and the consumables are even more expensive. When Yang Rui was working on test papers at school, he always organized mimeographs.

For ordinary materials, everyone also uses the method of copying, trying not to waste copy paper and other consumables.

Now, naturally, I couldn't wait to copy it.

Gu Qiang bravely printed a copy of the experimental design he wrote, and when he returned it to Yang Rui, he said: "In the future, I will find someone to copy these things. It is too expensive to print, and the paper costs a few cents a piece." of."

"It's not expensive to find someone?"

"For work-study students, 10 yuan a month is enough." Gu Qiang said as a matter of course.

10 yuan is not a lot. In many rural families, the annual cash income of a family is less than 120 yuan.

If students eat in the cafeteria, 10 yuan can guarantee a full stomach, and it will also be a little meaty.

Yang Rui ignored this problem, looked down at the experimental design of Gu Qiang's printed version, took off the cap of the pen while talking, and said: "If you can save office expenses, you need to save a little. Leave it to Director Xu to worry about it. Your time is yours. Don't waste it on this, the laboratory wants you to produce, not to save money."

"The saving is the output." Gu Qiang said in a low voice.

"If you can complete the subject of in vitro cultivation within two months, it will be the real output." Yang Rui's red pen crossed Gu Qiang's experimental design, and three circles were circled on the first line .

Gu Qiang was shocked by the red mark and surprised by Yang Rui's words, so he couldn't help repeating: "Two months?"

"The progress of Wang Liangcai's research group will also be accelerated." Yang Rui didn't say why, but kept everything in words.

The corner of Gu Qiang's mouth twitched twice, knowing that Yang Rui must have also been involved in Wang Liangcai's research.

Thinking about it, it is impossible for Yang Rui not to intervene.

From the beginning of the cloning project to the present, Wang Liangcai and Gu Qiang's group were busy competing and doing experiments, and Yang Rui never stopped thinking.

Now, he speaks out the results of his consideration, which will naturally help improve the progress of the project.

However, in two months, the subject of in vitro cultivation will be made from scratch, but Gu Qiang is not confident.

"I originally thought that there would be more time." Gu Qiang said cowardly.

Yang Rui smiled, picked up the pen again, and began to draw red circles.

On the first page alone, he drew more than a dozen red circles, almost beyond recognition.

Gu Qiang took a deep breath before lowering his head to see where Yang Rui had drawn.

The word "in vitro fertilized embryo" was circled and crossed out, and the oxygen consumption next to it was circled without other changes.

Later, Gu Qiang saw the circle drawn by Yang Rui for the word "densification" and the circle drawn for "culture solution".

Gu Qiang finally knew why Yang Rui asked him to make a copy.

After changing it like this, the entire experimental design is completely different.

If you don't leave a bottom plate, you really don't know the original specific content.

"I hope you can focus on the embryos after the blastocyst stage for research. However, the points you need to pay attention to are not only that." Seeing Gu Qiang watching, Yang Rui didn't seem to be too shocked, so he In addition to drawing circles and crosses, he gave an explanation by the way, saying: "The culture of cloned embryos, if you want to refer to the culture system of in vitro fertilized embryos, I don't agree."

Yang Rui tapped the red cross twice, and said: "Of course, the first standard is the judgment of the front-line researchers.

But from my personal understanding, if the original system is directly adopted without analysis, the result may not be saving time, but wasting energy. "

"Do you think cloned embryos will be different from normal embryos?" Gu Qiang couldn't help being a little surprised.

Leaving aside theological matters, scientists have a clear understanding of reproductive bodies such as embryos, and believe that cloned embryos should be the same as normal embryos. Otherwise, why cloning has caused so much controversy.

Therefore, in the process of culturing in vitro, Gu Qiang naturally adopted the same technical solutions as "test-tube babies" and "test-tube cows".

Yang Rui put the pen down and said: "I don't think that the cloned embryos will be fundamentally different from the normal embryos, but of course they are different. First of all, the cloned embryos we obtained are made with The oocytes were developed from the eggs, which are congenitally defective. Secondly, the oocytes have been soaked in various operating fluids. For example, as we know so far, immersion in sucrose solution will have adverse effects..."

Yang Rui said in a steady voice, "All in all, cloned embryos are abnormal embryos. If you use normal embryo culture methods, problems are likely to occur."

He said it was a guess, but in fact, Yang Rui was sure that something would go wrong.

This is also a difficulty in in vitro culture.

More problems will appear with poorly developed oocytes.

In short, in order to replace the nucleus of the egg cell, the operation of the scientist has caused great damage to it, and in vitro culture is to make up for this damage.

For example, if a weight of 5 catties is regarded as the minimum standard weight for a newborn, Newton, who weighed 2.7 at birth, would not have been able to survive. The modified cloned oocytes, which are equivalent to a child weighing 0.5 catties, are absolutely impossible to survive under natural conditions.

To allow it to develop normally, the problem it faces is not whether to feed it Sanlu milk, or whether it can afford imported milk powder.

Of course, if you can't afford imported reagents, the oocytes cannot be kept alive.

Gu Qiang was shocked by Yang Rui's detailed explanation, and couldn't help asking in a low voice: "Have you done in vitro training before?"

"When we were doing cattle embryo transfer, we did some preparatory research." Yang Rui said.

Gu Qiang was full of doubts. Although the embryo transfer of cattle also had the word "embryo", it was an order of magnitude different from the problem we are facing now.

Apart from other things, the expenses for in vitro culture of cloned sheep have to be spent in the millions. Basically all the remaining resources of Gu Qiang's group had to be transferred.

What about bovine embryo transfer? Even with the hidden investment of China Animal Husbandry and other companies, the total sum is not so much.

Gu Qiang licked his lips and asked, "When you do cow embryo transfer, you want to do cloning, right?"

Yang Rui glanced at Gu Qiang with a smile, but did not answer. He tapped on the experimental design with a pen and said, "The next step is the details of the experiment. I have some ideas for your reference?"

"You said." Gu Qiang's attitude was positive.

"From the data point of view, adding various additives to the basic nutrient solution should also be able to improve the development of cloned embryos." Yang Rui did not elaborate on the data, and said: "We start from cell embryos, we need to Facing a block in embryonic development."

Embryo development block refers to the phenomenon that the embryo develops to the four-cell stage and stops developing. This is mainly related to the activation of the embryonic genome.

Without the Genome Project today, it would take many and varied attempts to break that block.

Yang Rui didn't go into details, but drew two circles in Gu Qiang's experimental design, saying: "Adding oviduct fluid to the culture medium, and co-cultivating with oviduct epithelial cells, I am more in favor of these two options. You You can try it first."

"Okay." Gu Qiang nodded repeatedly.

"The culture medium is also an important point. You have to make good experimental records, complete experimental records." Yang Rui specially reminded.

A clear formula is very important for the culture medium.

Of course, scientists today don't realize this because no one has started cloning yet.

In later generations, if we say which part of cloning is the most complicated and has the most threshold, it is not the technology of peeling eggs, but the preparation of embryo culture medium.

Yang Rui thought for a while, and decided to help Gu Qiang reduce a little burden, and then said: "From my personal feeling, I think the more promising additive of culture medium, fetal bovine serum, we have used before. Epidermal growth factor , This is a new thing, you should try it. Insulin growth factors, antioxidants, anthocyanins, I think they can all be considered. In addition, oxygen is also very important.”

"Okay." Gu Qiang recorded it simply, without any clue for the time being.

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