Rebirth of the God-level Xueba

From Wang Liangcai's point of view, Yang Rui's operation is hard to say the bright spot.

He is a student from Tong's lineage. He studied genetics as an undergraduate, and trained with scholars from the Institute of Hydrology of the Chinese Academy of Sciences for many years. kinds of items. As far as egg peeling skills are concerned, Wang Liangcai is world-class, far surpassing Peking University's so-called egg peeling masters, and Yang Rui's skills are even less in his eyes.

However, Wang Liangcai wanted to write down every movement performed by Yang Rui.

In just a few tens of minutes—roughly equivalent to the time spent watching a Wong Kar Wai movie in a movie theater and waking up twice, Yang Rui has basically completed the denuclearization operation.

The denuclearization operation is not difficult, especially for researchers from the Institute of Fisheries, it is the basic of the basics.

But a player like Yang Rui, who only failed once and successfully completed the denuclearization operation, is the real surprise.

In other words, Yang Rui's operation technique is not worth mentioning, but he used such an operation to complete the denucleation operation with ease, which is the reason why Wang Liangcai paid attention.

In clonal cell manipulations, a total of two enucleation operations are required.

The first enucleation operation is to remove the nucleus from the sheep oocyte. Because the nucleus is not used in the later stage, the main point of the first enucleation operation is to suck out the cytoplasm of the cell as little as possible, and at the same time, suck the nucleus completely.

The second enucleation operation is for ordinary cells.

The mammary gland cells of the sheep are used to clone the sheep. The main point of the operation at this time is to completely suck out the cytoplasm of the mammary gland cells of the sheep. As for the mammary gland cells themselves, they will be discarded later, so it does not matter.

In general, the two steps actually require refinement, and there are very few shortcomings that can be tolerated. The best state must be to completely separate the nucleus from the cell itself.

Otherwise, if the nucleus left by the oocyte participates in development in the later stage, it is hard to say whose sheep will be born.

If it is used for cloning, you originally wanted to clone Dolly the sheep, but in the end Dolly carried the beautiful genetics, so this kind of cloning is not clear.

Not exactly a sensational clone, either.

From the very beginning, Wang Liangcai was very clear about the purpose of this part of the operation. However, in order to suck out the nucleus of the oocyte, he had to suck out more cytoplasm. As a result, the remaining cytoplasm was not enough to develop, so it was declared a failure.

Gu Qiang's operation is actually the same. In this step, the cytoplasm is too easily sucked out.

Instead, it is the second step. It does not matter how much cytoplasm is lost, only the integrity of the nucleus is required, which greatly reduces the technical requirements of the experimenter.

Yang Rui's newly developed technology is different.

Although the goal of the operation has not changed, it is still to suck out all the nuclei and as little cytoplasm as possible.

However, Yang Rui's operation lowered the requirements a bit. Not only did he not pursue the complete suction of the cell nucleus, but he intentionally crushed the cell nucleus, and then sucked out the fragments little by little.

Complete aspiration of the nucleus can certainly meet the requirements, but it is too difficult.

Moreover, the nucleus is not a hard solid, it is more like a balloon swimming in a viscous liquid, if it is completely sucked out, it is inevitable to increase the suction force, thereby sucking out more cytoplasm, in addition, When the balloon comes out, it will inevitably bring out more cytoplasm. Sometimes it brings out too much, and it directly declares that the experiment has failed. Sometimes it brings out less, and there is a lot of luck.

If it is sucked through, of course it will cause more trouble.

Think about a balloon being broken in the liquid, the gas in the balloon is still floating out, forming bubbles one by one in the liquid, and how troublesome it is to suck them out one by one.

However, trouble is troublesome, but it is a troublesome job that can be done. In addition to careful and cumbersome operations, it is much easier to suck up fragments than a whole balloon.

Sometimes, if the core suction needle is well controlled, it doesn't even need to suck much fragments, and the task can be completed after three or five negative pressure suctions.

After Wang Liangcai discovered this, he couldn't move forward.

Immediately afterwards, Wang Liangcai discovered that suction crushing was only the smallest point among the new technologies developed by Yang Rui.

Using the spindle to control the amount of absorption is the most powerful part, it can be said to be exquisite.

As the name suggests, the nucleus suction needle is used to absorb the nucleus. How small is its needle tip? Basically, you may not be able to see clearly under a microscope with ordinary magnifications.

Therefore, when operating the core suction needle, a slight vibration will make it swing violently.

You can imagine the feeling of holding the rope in your hand when threading a needle. The cells are much smaller than the eye of a needle, and the hand trembles a lot, not to mention sucking the nucleus, it is normal to puncture the cells.

Yang Rui's level is weaker than Wang Liangcai's. He is also a certain level of experimenter, and he still accidentally punctured an oocyte, which can measure the difficulty.

However, when the core-absorbing needle was pressed against the spindle, the trembling disappeared naturally.

It's like holding a long stick in your hand, the head of the stick will vibrate uncontrollably, but when you poke the stick against the wall, the head of the stick will not vibrate.

In addition to being able to control the vibration of the core suction needle and increase accuracy, another benefit is to control the back and forth vibration of the core suction needle.

That is to say, when the core suction needle pokes the spindle, it seems to add a fault-tolerant device out of thin air, making the operation easier at once.

It's easy to say, but it's not easy to think of using this trick.

The hardest part is finding the correct poke position for you.

The spindle is also a very important part of the cell, and it is not easy to poke it. It is naturally tougher, but some parts are too tough, which will hinder absorption, and some parts are unexpectedly fragile, and it is easy to poke.

The remaining spindles that can be poked should preferably be in a relatively easy-to-operate position.

Watching Yang Rui's movements, Wang Liangcai went back and forth in his mind, wishing he could master the new method immediately and try it later.

At this point, Yang Rui could not satisfy Wang Liangcai's request.

He did it slowly, and it was a new method that he had only tried a few times. The operation was so clumsy that people wanted to jump, just like a child who just learned how to shoot a ball doing a three-step layup. It is ugly when there is no foul Die, not to mention more fouls than walks.

"Professor Yang, I understand a little bit, otherwise, let me try to operate it?" Wang Liangcai said in a low voice.

Yang Rui raised his eyelids and said, "Why, do you think my operation is not good?"

Wang Liangcai coughed twice, and said against his will: "How can it be, your operation is no problem."

"I also think, wait a minute, I'll talk about it after I've de-cored it." Yang Rui really felt that he did a good job, but it was not as good as Wang Liangcai's operation, and it was still improved compared to the previous operation.

Naturally, he ignored the difficulty gap between old technology and new technology.

Wang Liangcai was in a hurry, and he couldn't rush, so he could only watch obediently.

If Wang Liangcai had added two catties of bear cell nuclei, he would have jumped up to tell the truth. The same conditions also apply to other researchers in the laboratory.

As time passed, Su Fan, Gu Qiang, Tian Bing and others silently gathered in front of the test bench.

Everyone knows Yang Rui's experimental ability.

As for how long an oocyte can withstand the ravages, everyone has some idea.

Normally, if you give Yang Rui an oocyte, even if he doesn't pierce it, he won't be able to play for two hours.

No matter how long it takes, the progress of the experiment will be deep.

Think about Gu Qiang's luck to complete the nuclear injection, and Wang Liangcai sleeping in the laboratory for several weeks before barely catching up with the progress...Yang Rui couldn't just take Gu Qiang's luck and finish it for a few hours at a time.

The more researchers you pay attention to, the more curious other researchers will become.

In the afternoon, the experimental platform where Yang Rui was located was already full of people.

Yang Rui didn't notice anything.

Unlike the boring experimentation of other people, he is trying a new method, a bit like playing a new game, and he is always addicted to it at the beginning.

Gulp.

Someone's stomach rang.

Yang Rui raised his head in a daze, and rubbed his sore neck, only to notice all kinds of strange eyes around him, as well as the moving heads.

"Hey, don't be crowded, aren't you guys hot?" Yang Rui drove the crowd away and said, "Be careful, don't pollute the test bench."

Everyone hurriedly took two steps back to make room.

"It's almost eight o'clock. Those who haven't eaten can go to eat. I still have several hours here." Yang Rui touched his stomach and decided to wait before eating. Stomach can wait, but cells can't.

Everyone nodded and persuaded each other: "Old Tian, ​​you have a bad stomach, go eat quickly."

"Xiao Lei, don't hold back, go and eat, go and eat."

"Su Fan, today I have your favorite braised pork. Be quick and slow."

"Then why don't you go." Su Fan pouted, not caring about the persuasion of others.

In fact, no one wants to go at all.

How rare is it to see a demonstration of a new technology, and it was the Nobel Prize-winning Yang Rui who was doing the demonstration. When I said later, "When I watched Professor Yang do egg cell enucleation", the grandeur naturally rose.

Yang Rui didn't say much, he was here to demonstrate the technology, so he couldn't drive people away.

He rubbed his belly and continued to work, and the others gathered together as usual, watching and discussing in a low voice.

In a laboratory, there is always a difference in level, especially when faced with a specific subject, there are always some people who have mastered it thoroughly, and there are always some people who are not very good at it.

At this time, everyone's attention is different.

The so-called layman watches the excitement, and the expert watches the way, and it is the same in the same laboratory.

Even if he just looked at everyone's notebooks, Wang Liangcai remembered more than others.