Rebirth of the God-level Xueba

Wang Liangcai still came very early, in fact, he came earlier than before.

The main machine time was adjusted to morning, so that Wang Liangcai no longer had to worry about organizing time and vacating space, etc., and could concentrate on completing the experiment. The most important thing is that what he can do in the early morning is not only the experiment preparation and preliminary experiments, but there is no problem in conducting formal experiments.

In order to make the most of this time, Wang Liangcai went to bed after dinner after watching only one episode of Dream of the Red Chamber. Faced with the only entertainment, he was able to be cruel because this project was so important.

Wang Liangcai is very fortunate that he was selected for this national project.

How important is the sheep cloning project?

For scholars of genetics, this is basically the No. 1 project in Tianzi.

If the cloning technology matures, all breeding and breeding will be a thing of the past. The history of living in tents and forcing cattle, sheep and horses to mate will be gone forever, just like blacksmiths are to steam engines. They will still have jobs, but more of them are just unimportant leftover jobs, or for saving money, or for art, anyway, it is meaningless behavior.

However, no matter how important cloning is, Wang Liangcai would not dare to start the project himself if he cloned another eighty pairs of bear hearts and leopard galls.

Needless to say, even Wang Liangcai's laboratory cannot afford an inverted phase-contrast microscope.

Not to mention, such a project is bound to compete with foreign counterparts.

Even if you don't say what you are doing, those who are engaged in genetic science abroad are approaching this goal step by step.

As long as he thinks about the world's leading genetic engineering laboratories, Wang Liangcai feels the pressure doubled during the period when he may set foot in it.

That is to say, in Yang Rui's genetic engineering laboratory, Wang Liangcai's mood can be calmer, and he can do experiments with peace of mind, without having to think about so many miscellaneous things.

For Wang Liangcai, this is the state he has been longing for for a long time.

In comparison, watching one less episode of Dream of the Red Chamber every day, going to bed a few hours earlier, and waking up a few hours earlier is nothing.

Who did not do this before taking the college entrance examination.

Teenagers can muster up the courage and perseverance to do things, but middle-aged people can't do it?

That is the more you live, the more you come back.

Driven by Wang Liangcai, many researchers in the laboratory came earlier than usual. The most obvious one is Su Fan.

Now, Su Fan is an old man in the ion channel laboratory.

He was one of the first to get into the ion channel laboratory when Yang Rui dug Mavericks, and later he opened his own research group, specializing in cloning-related research.

It should be said that his research group still has a very good output, and it has given considerable help when doing bovine embryo transfer in the Genetic Engineering Laboratory of Haidian District.

Afterwards, Su Fan joined the G protein-coupled receptor project and undertook a lot of work.

Regardless of the evaluation standards of any period, Su Fan can be regarded as an accomplished researcher.

However, the most fearful thing in everything is comparison.

Su Xiankai, who joined the ion channel laboratory at the same time as Su Fan, started with a dog and independently started the G protein-coupled receptor project. After that, there are three in command and four in command.

Others might be able to bear it, but Su Fan couldn't bear it at all.

Su Fan, who graduated from Beiyan Agricultural College, has always been very sensitive to differential treatment. Maybe it's because he has suffered more discrimination, so Su Fan has been very concerned about other people's opinions and arrangements since a long time ago.

Educational discrimination in academia is the most blatant discrimination, and to break through its shackles, education must be separated from ability.

Emphasizing one's ability to prove that education is only caused by playing around when you were young and ignorant is a common method when you are discriminated against by education.

So did Su Fan.

It wasn't until the previous unit tore away the fig leaf of ability-determined job titles that Su Fan switched to the ion channel laboratory.

In the ion channel laboratory,

Su Fan lived a comfortable life for a period of time, but psychologically speaking, his pressure has not eased.

The prefix of the ion channel laboratory is Peking University, therefore, most of the researchers Su Fan saw in the laboratory were researchers with Peking University degrees. Not to mention Yang Rui and Xu Zhengping, later Jiao Yangping, Wang Sisheng and others were from Tsinghua University, and then Professor Man Hai, Feng Junming and Tao Xuelin were also from Peking University, which touched Su Fan a lot.

Some time ago, the work was too intense, so Su Fan didn't have time to think about all this carefully.

After being dispatched to the genetic engineering laboratory, Su Fan's heart of comparison couldn't be suppressed no matter what.

Especially compared with Gu Qiang, Su Fan disagrees with the latter even more.

Gu Qiang did not graduate from a prestigious school, so why did he lead a research group?

Although Gu Qiang has completed many experiments in a row, Su Fan still wants to compete with him.

Of course, cultural people can't challenge directly, that would be too ugly. Su Fan decided to learn from Wang Liangcai, taking a step ahead of Gu Qiang, and working on the subject of cell enucleation.

It is said to be studying, but it is actually helping Wang Liangcai.

If not, Su Fan is not confident that he can complete the work of enucleation.

Unlike Wang Liangcai who has been trained for a long time, Su Fan himself has not received the practice of "egg peeling". Beiyan Agricultural College does not teach this, and after he joined the work, he could not find anyone to learn it. Therefore, conducting experiments independently is asking for trouble.

Su Fan had the mentality of stealing the teacher, but also had the attitude of helping, and began to help Wang Liangcai. He wants to learn for a while before considering whether to restart the stove or open up a new path.

However, the technology mastered by Wang Liangcai obviously greatly exceeded Su Fan's expectations.

Everyone is peeling eggs. Gu Qiang's egg peeling ability can basically be said to be enough, nothing more than peeling off.

Wang Liangcai's standard technique has been practiced for many years, and there is really too much to learn.

After running with Wang Liangcai for a few mornings, Su Fan got up earlier and earlier.

The interaction between the two fell into Gu Qiang's eyes without any surprise.

It would be a bit false to say that there is no rush at all. Members of the same research group also have competition. At the very least, when a paper is published in the future, everyone will have to fight for one.

But there's no use in worrying. Tong Dizhou's apprentices and grandchildren are all egg-stripping stars. Although Gu Qiang, who was born in Shanda University, has strong experimental skills, he really has no advantage in terms of egg-stripping.

Day by day passed, and seeing Wang Liangcai and Su Fan cooperate more and more tacitly, and the two also transitioned from the nucleation experiment to the denuclearization experiment, Gu Qiang couldn't sit still anymore.

"Director Yang, I want to change an experiment idea." Gu Qiang's research team can only conduct experiments within the specified range, and Yang Rui's consent is required to change the idea.

Yang Rui is well aware of the recent atmosphere in the laboratory, but he is so happy. Those who do scientific research do it for three hours a day, eight hours a day, and eighteen hours a day. It makes a big difference whether there is competition or not.

Healthy competition in the laboratory couldn't be better, Yang Rui asked without any surprise: "How do you want to change?"

"I want to do a one-stop experiment, and I don't want to do it step by step." Gu Qiang also used his trump card.

The steps of cloning are actually quite simple. The core part is to enucleate the egg cells of one sheep, stuff them into new egg cells, and then stuff them into the body of another sheep. The lamb born is cloned. sheep.

Although there are still many steps before and after, such as superovulation, such as taking care of pregnant sheep, etc., any step is indispensable. However, there are actually not many core steps.

Doing it step by step is not only a practice, but also a way to find a higher success rate.

Like the last step, you have to let the ewe give birth to a healthy lamb. If you can't find ways to increase the success rate, the previous steps are not all wasted.

When the Scots were cloning sheep, they failed at the last step three times, and each time they were a few months old. This hard work can be realized after thinking about it.

However, formal methods are not particularly suitable for Gu Qiang.

He is not an experimental biologist with a good theoretical level, more like technicians in foreign laboratories. Although the latter also plays with theory, they are all playing with specific operations. The difference between the two is like the difference between an automobile engineer and a car repairman.

An excellent car repairman is sometimes better than an automobile engineer to modify a car or something. However, if you want to develop new technologies step by step and improve the technical indicators of each component of the automobile, you still need an automobile engineer to do it.

Gu Qiang also knows his own shortcomings. After working for a while, it is difficult for him to improve the accuracy rate in a single session. It is not a problem of experimental techniques, but a problem of small ideas of small experiments.

Therefore, Gu Qiang simply proposed a one-stop cooperation.

Yang Rui couldn't help being silent, he also knew about Gu Qiang's problems, but the reason why he did it step by step was a formal method, because when he did it step by step, the overall success rate was higher, and compared with a one-stop process, he spent less resources , more results are obtained, the repeatability rate is higher, and more importantly, it saves time.

It takes a few months for a sheep to conceive. If you go through it one by one, it will take a year if you fail once, and there are so many failures.

However, considering Gu Qiang's strong luck, Yang Rui hesitated.

If according to formal academic judgment standards, Gu Qiang's level is simply not as good as Wang Liangcai's.

Although Gu Qiang is top-notch in China in terms of experimental ability, but Wang Liangcai is top-notch in the Chinese Academy of Sciences. Domestically speaking, he is better than him in technology, and his age is beyond the standard. Those younger than him have lower theoretical level and experience. far from...

It can only be said that Gu Qiang shot himself in the foot with a stone, and he called Wang Liangcai's name when he opened his mouth. He didn't expect his skills to fully cover him.

Except "strong luck".

Thinking of this word again, Yang Rui couldn't help but look up at Gu Qiang, who gave an awkward yet polite smile.

"If you change a dragon, the funding will change. Have you prepared?" Seeing that Gu Qiang has made breakthroughs in several scientific researches, Yang Rui was relieved.

Gu Qiang said quickly: "I have finished writing the report."

"Where is it?" Yang Rui glared at him, why did he say something was not delivered.

Gu Qiang hurried back to get it, turned back and handed it to Yang Rui, and said with a dry smile, "I didn't expect you to agree."

"You mean I shouldn't agree?"

"No, no..." Gu Qiang smirked again. He originally thought that Yang Rui would have to bargain, for example, splitting one dragon into two dragons or something, which would reduce costs and risks, but he didn't expect that Yang Rui would not ask for a bargain.

"The expected cost is 6 million RMB, plus 300,000 U.S. dollars?" Yang Rui laughed when he saw the final figures in the report.

This is in his laboratory. Outside of Yang Rui's laboratory, there are only a handful of laboratories in the country that can provide this funding.

Of course, Yang Rui's laboratory itself is one of the few high-end laboratories in China, but this amount of funding is still very exaggerated.

Previously, when Yang Rui was working on a large project, the total funding would fluctuate around this figure.

It can be said that in China, a budget of 10 million yuan is the ceiling. Those who can reach this figure do not depend on the achievements and reputation. You can achieve great results, and at the same time have great reputation, trust, and good experience. , You have to find the right project to be eligible to compete for such a funding value.

Gu Qiang is far away from such a qualification.

With a wave of his hand, Yang Rui removed the 0 after 6 million and 300,000, and said: "600,000 plus 30,000 US dollars, there is almost 1 million funds, which must be enough to buy consumables."

"I still want to buy a few more instruments..."

"Let's talk about it after making something." It is impossible for Yang Rui to approve more funds for Gu Qiang alone.

If you want to say, 1 million is too much.

He is only in charge of a research team, not a project team, and all the honors belong to the Lord, so he cannot decide on major expenses such as the purchase of instruments.

However, if Gu Qiang can compete with Wang Liangcai this time and make things in one go, it will be different.

It is actually a very good thing for Yang Rui to open another project team in the laboratory.

Scholars can't be emperors, at most they can become the state of Beiyang warlords. There are factions in the warlords, which is not only normal, but also healthy. On the contrary, those who wanted to be a dictatorship ended up ruined.

Gu Qiang didn't think as much as Yang Ruiyuan did. He was very satisfied with the funding of 600,000 + 30,000 US dollars. He gritted his teeth and said, "Look at the progress on my side. I'm sure I'll come from behind."

"Is this behind?"

"No...it's not considered backward." Gu Qiang quickly defended: "My success rate of displaying the core is almost 50%, and the success rate of de-nucleation is not low. I just think that if you do it step by step It's so boring..."

His voice is getting smaller and smaller, because his success rate is lower than Wang Liangcai's, and the numbers cannot be disputed.

"Director Yang, shall I go back first?" Seeing that Yang Rui was still reading his poorly prepared report, Gu Qiang was inexplicably guilty.

"Wait." Yang Rui called him slowly, and said, "Are you going to use embryonic nuclei from the blastocyst stage for your nuclear injection?"

"Yes, do you think the blastocyst stage is not suitable?" Gu Qiang asked humbly.

During the development of embryos, there are multiple stages, and it is very common to use cells from the blastocyst stage.

Yang Rui shook his head and said, "I have no problem with the blastocyst stage, but I have a problem with the embryo."

"Would you like a different kind of sheep?"

Yang Rui shook his head again, and said, "My idea is to try non-fertility cells, for example, breast cells and other somatic cells?"

Gu Qiang couldn't stand still: "How can the nucleus of somatic cells be used to inject nuclei?"

"How do you know if you don't try it?" Yang Rui said, "Didn't I mention it at the project briefing meeting?"

"I think it's a long-term plan." Gu Qiang was terrified, and more of a denial.

Yang Rui said: "Since you have decided to do a one-stop experiment, then don't wait."

"This..." Gu Qiang regretted it in his heart.

Before the birth of cloned sheep, the specificity and omnipotence of biological cells were listed as important theories, including in Chinese biology textbooks.

To put it simply, according to the current understanding, a cell is totipotent during the embryonic period, that is, it can do anything in the future.

The cells that differentiate into skin tissue, blood, and heart are specific, and it is theoretically believed that they cannot grow in reverse, unlike embryos, which can differentiate whatever they want.

Taking fertility as an example, it means that there are only a few with fertility, and the others cannot be used for fertility.

It is this point that the cloning sheep Dolly really breaks through.

Perhaps for ordinary people, what kind of cell nucleus is not injected? But for biologists, it's a sea change.

It is not an exaggeration to say that the biological building has collapsed.

Although the biological building collapses every year, but this time it is indeed more serious.

Therefore, although Yang Rui began to expound his ideas from the beginning of the project, including Gu Qiang and others, they still regarded it as science fiction.

When fantasies come into reality, sometimes it is beautiful, but sometimes it is worse than a nightmare.